62.2 Folate

62.2.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

Prolactin
Chisq Df Pr(>Chisq)
(Intercept) 250.7924480 1 <0.0001 ***
Study 3.2136720 1 0.07 ns
f39_gdm_new 0.5754347 1 0.4 ns
BMI 3.3368238 1 0.07 ns
Ethnicity_cauc 105.4219556 1 <0.0001 ***
f10c_any_cig_1st_vst 49.1590682 1 <0.0001 ***
GA_samp 164.6323095 1 <0.0001 ***
Study:f39_gdm_new 1.8652579 1 0.2 ns
HPL
Chisq Df Pr(>Chisq)
(Intercept) 150.8759535 1 <0.0001 ***
Study 7.3986291 1 0.007 **
f39_gdm_new 0.4357432 1 0.5 ns
BMI 236.2094019 1 <0.0001 ***
Ethnicity_cauc 5.5165373 1 0.02 *
f10c_any_cig_1st_vst 0.9176455 1 0.3 ns
GA_samp 2601.9722143 1 <0.0001 ***
Study:f39_gdm_new 1.4845286 1 0.2 ns
GH2
Chisq Df Pr(>Chisq)
(Intercept) 251.096669 1 <0.0001 ***
Study 1.107622 1 0.3 ns
f39_gdm_new 1.386104 1 0.2 ns
BMI 87.622496 1 <0.0001 ***
Ethnicity_cauc 3.246832 1 0.07 ns
f10c_any_cig_1st_vst 1.039153 1 0.3 ns
GA_samp 1032.266869 1 <0.0001 ***
Study:f39_gdm_new 3.376951 1 0.07 ns
Insulin
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 21.326 21.326 <0.0001 ***
3 f39_gdm_new 1 Inf 33.726 33.726 <0.0001 ***
4 BMI 1 Inf 330.722 330.722 <0.0001 ***
5 Ethnicity_cauc 1 Inf 25.046 25.046 <0.0001 ***
6 f10c_any_cig_1st_vst 1 Inf 3.158 3.158 0.08 ns
7 GA_samp 1 Inf 2.506 2.506 0.1 ns
2 Study:f39_gdm_new 1 Inf 4.089 4.089 0.04 *
HOMA.IR
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 36.051 36.051 <0.0001 ***
3 f39_gdm_new 1 Inf 32.027 32.027 <0.0001 ***
4 BMI 1 Inf 316.807 316.807 <0.0001 ***
5 Ethnicity_cauc 1 Inf 24.882 24.882 <0.0001 ***
6 f10c_any_cig_1st_vst 1 Inf 2.618 2.618 0.1 ns
7 GA_samp 1 Inf 1.602 1.602 0.2 ns
2 Study:f39_gdm_new 1 Inf 4.361 4.361 0.04 *
GLUC3_mmol.L
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 53.182 53.182 <0.0001 ***
3 f39_gdm_new 1 Inf 4.345 4.345 0.04 *
4 BMI 1 Inf 11.449 11.449 0.0007 ***
5 Ethnicity_cauc 1 Inf 0.718 0.718 0.4 ns
6 f10c_any_cig_1st_vst 1 Inf 0.028 0.028 0.9 ns
7 GA_samp 1 Inf 0.278 0.278 0.6 ns
2 Study:f39_gdm_new 1 Inf 1.558 1.558 0.2 ns

62.2.2 Model summary

Ratio of Geometric means (95% CI)

Prolactin
Var Effect P
(Intercept) 2.75 (2.39, 3.12) <0.0001
Folate -0.00 (-0.00, 0.00) 0.12
f39_gdm_newGDM -0.11 (-0.35, 0.13) 0.36
StudySTOP -0.08 (-0.21, 0.05) 0.23
GA_samp 0.14 (0.12, 0.16) <0.0001
Folate:f39_gdm_newGDM 0.00 (-0.00, 0.00) 0.75
f39_gdm_newGDM:StudySTOP 0.16 (-0.07, 0.38) 0.17
Folate:StudySTOP 0.00 (-0.00, 0.00) 0.10
HPL
Var Effect P
(Intercept) 0.65 (0.46, 0.84) <0.0001
Folate -0.00 (-0.00, 0.00) 0.58
f39_gdm_newGDM -0.11 (-0.23, 0.01) 0.07
StudySTOP 0.10 (0.03, 0.17) 0.004
GA_samp 0.29 (0.28, 0.30) <0.0001
Folate:f39_gdm_newGDM 0.00 (-0.00, 0.00) 0.28
f39_gdm_newGDM:StudySTOP 0.06 (-0.05, 0.17) 0.31
Folate:StudySTOP -0.00 (-0.00, 0.00) 0.89
GH2
Var Effect P
(Intercept) -2.57 (-2.82, -2.32) <0.0001
Folate -0.00 (-0.00, 0.00) 0.37
f39_gdm_newGDM -0.17 (-0.34, 0.00) 0.05
StudySTOP -0.01 (-0.11, 0.08) 0.77
GA_samp 0.26 (0.25, 0.28) <0.0001
Folate:f39_gdm_newGDM 0.00 (-0.00, 0.00) 0.65
f39_gdm_newGDM:StudySTOP 0.17 (0.00, 0.33) 0.05
Folate:StudySTOP -0.00 (-0.00, 0.00) 0.89
Insulin
Var Effect P
(Intercept) 2.45 (1.99, 2.91) <0.0001
Folate -0.00 (-0.00, 0.00) 0.56
f39_gdm_newGDM 0.94 (0.44, 1.44) 0.0002
StudySTOP -0.17 (-0.36, 0.02) 0.07
GA_samp 0.02 (-0.01, 0.05) 0.24
Folate:f39_gdm_newGDM -0.00 (-0.01, 0.00) 0.27
f39_gdm_newGDM:StudySTOP -0.42 (-0.98, 0.13) 0.14
Folate:StudySTOP -0.00 (-0.00, 0.00) 0.24
Folate:f39_gdm_newGDM:StudySTOP 0.00 (-0.00, 0.01) 0.38
HOMA.IR
Var Effect P
(Intercept) 1.09 (0.60, 1.58) <0.0001
Folate -0.00 (-0.00, 0.00) 0.54
f39_gdm_newGDM 1.08 (0.52, 1.65) 0.0002
StudySTOP -0.32 (-0.51, -0.12) 0.002
GA_samp 0.01 (-0.02, 0.04) 0.39
Folate:f39_gdm_newGDM -0.00 (-0.01, 0.00) 0.18
f39_gdm_newGDM:StudySTOP -0.51 (-1.13, 0.10) 0.10
Folate:StudySTOP -0.00 (-0.00, 0.00) 0.25
Folate:f39_gdm_newGDM:StudySTOP 0.00 (-0.00, 0.01) 0.33
GLUC3_mmol.L
Var Effect P
(Intercept) 1.73 (1.63, 1.82) <0.0001
Folate -0.00 (-0.00, 0.00) 0.67
f39_gdm_newGDM 0.16 (0.03, 0.30) 0.02
StudySTOP -0.15 (-0.18, -0.11) <0.0001
GA_samp -0.00 (-0.01, 0.00) 0.44
Folate:f39_gdm_newGDM -0.00 (-0.00, -0.00) 0.03
f39_gdm_newGDM:StudySTOP -0.07 (-0.19, 0.04) 0.20
Folate:StudySTOP 0.00 (-0.00, 0.00) 0.61

62.2.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Serum folate concentration (nM) and may be misleading when interaction term is involved.

Prolactin
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 97.77404 7.328399 86.89088 84.24089 113.4813
STOP non-GDM 95.21737 6.830184 73.12151 82.53345 109.8506
SCOPE GDM 88.89601 11.031476 534.09053 69.66477 113.4361
STOP GDM 101.42073 8.859190 156.34178 85.34803 120.5202
HPL
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 89.48653 2.397042 155.4415 84.87464 94.34902
STOP non-GDM 98.99553 2.442050 113.5757 94.27396 103.95357
SCOPE GDM 82.59212 4.500140 823.2588 74.21497 91.91486
STOP GDM 96.91737 3.552318 432.8746 90.18100 104.15695
GH2
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 2.358518 0.0675800 372.7572 2.229306 2.495219
STOP non-GDM 2.316497 0.0571383 254.7097 2.206662 2.431798
SCOPE GDM 2.022779 0.1546622 1400.9025 1.741041 2.350107
STOP GDM 2.343339 0.1019796 804.3136 2.151473 2.552315
Insulin
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 14.21264 0.7009927 Inf 12.90303 15.65516
STOP non-GDM 11.34214 0.4379906 Inf 10.51538 12.23391
SCOPE GDM 32.57401 5.7010512 Inf 23.11508 45.90365
STOP GDM 18.90218 1.4998809 Inf 16.17966 22.08281
HOMA.IR
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 3.444485 0.1804177 Inf 3.108418 3.816885
STOP non-GDM 2.366071 0.0958502 Inf 2.185472 2.561593
SCOPE GDM 8.779249 1.7041093 Inf 6.001138 12.843433
STOP GDM 4.100215 0.3501282 Inf 3.468329 4.847223
GLUC3_mmol.L
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 5.431002 0.0507757 Inf 5.332390 5.531438
STOP non-GDM 4.701537 0.0367122 Inf 4.630130 4.774045
SCOPE GDM 6.129766 0.3417906 Inf 5.495177 6.837639
STOP GDM 4.927915 0.0698577 Inf 4.792881 5.066753

62.2.4 Comparisons

62.2.4.1 Within Groups

Effect for every 50 unit increase in Serum folate concentration (nM).
Hormone Contrast Study f39_gdm_new Ratio of Geometric means (95% CI) P-value
Prolactin Folate40.2 / Folate39.2 SCOPE non-GDM 0.999 (0.998, 1.000) 0.1
Prolactin Folate40.2 / Folate39.2 STOP non-GDM 1.000 (0.999, 1.001) 0.5
Prolactin Folate40.2 / Folate39.2 SCOPE GDM 0.999 (0.997, 1.002) 0.6
Prolactin Folate40.2 / Folate39.2 STOP GDM 1.001 (0.998, 1.003) 0.5
HPL Folate40.2 / Folate39.2 SCOPE non-GDM 1.000 (0.999, 1.000) 0.6
HPL Folate40.2 / Folate39.2 STOP non-GDM 1.000 (0.999, 1.000) 0.4
HPL Folate40.2 / Folate39.2 SCOPE GDM 1.001 (0.999, 1.002) 0.5
HPL Folate40.2 / Folate39.2 STOP GDM 1.000 (0.999, 1.002) 0.5
GH2 Folate40.2 / Folate39.2 SCOPE non-GDM 1.000 (0.999, 1.001) 0.4
GH2 Folate40.2 / Folate39.2 STOP non-GDM 0.999 (0.999, 1.000) 0.2
GH2 Folate40.2 / Folate39.2 SCOPE GDM 1.000 (0.998, 1.002) 1.0
GH2 Folate40.2 / Folate39.2 STOP GDM 1.000 (0.998, 1.002) 0.9
Insulin Folate40.2 / Folate39.2 SCOPE non-GDM 0.999 (0.997, 1.001) 0.6
Insulin Folate40.2 / Folate39.2 STOP non-GDM 0.998 (0.997, 0.999) 0.002
Insulin Folate40.2 / Folate39.2 SCOPE GDM 0.997 (0.992, 1.001) 0.1
Insulin Folate40.2 / Folate39.2 STOP GDM 0.998 (0.995, 1.001) 0.2
HOMA.IR Folate40.2 / Folate39.2 SCOPE non-GDM 0.999 (0.997, 1.001) 0.5
HOMA.IR Folate40.2 / Folate39.2 STOP non-GDM 0.998 (0.997, 0.999) 0.002
HOMA.IR Folate40.2 / Folate39.2 SCOPE GDM 0.996 (0.991, 1.001) 0.09
HOMA.IR Folate40.2 / Folate39.2 STOP GDM 0.997 (0.994, 1.001) 0.1
GLUC3_mmol.L Folate40.2 / Folate39.2 SCOPE non-GDM 1.000 (1.000, 1.000) 0.7
GLUC3_mmol.L Folate40.2 / Folate39.2 STOP non-GDM 1.000 (1.000, 1.000) 0.7
GLUC3_mmol.L Folate40.2 / Folate39.2 SCOPE GDM 0.999 (0.998, 1.000) 0.05
GLUC3_mmol.L Folate40.2 / Folate39.2 STOP GDM 0.999 (0.998, 1.000) 0.01

62.2.4.2 Between Groups

Below are comparisons of the differences in the change in Serum folate concentration (nM) between groups. I.e. whether the change in Serum folate concentration (nM) are different between groups.

Prolactin
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 1.002779 0.0017041 2094.601 0.9994426 1.006127 0.103 ns
STOP/SCOPE (non-GDM) 1.001389 0.0008509 2094.601 0.9997213 1.003059 0.103 ns
STOP/SCOPE (GDM) 1.001389 0.0008509 2094.601 0.9997213 1.003059 0.103 ns
GDM/non-GDM (SCOPE) 1.000395 0.0012409 2037.473 0.9979647 1.002832 0.750 ns
GDM/non-GDM (STOP) 1.000395 0.0012409 2037.473 0.9979647 1.002832 0.750 ns
HPL
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 0.9998737 0.0009085 1994.048 0.9980936 1.001657 0.889 ns
STOP/SCOPE (non-GDM) 0.9999368 0.0004543 1994.048 0.9990463 1.000828 0.889 ns
STOP/SCOPE (GDM) 0.9999368 0.0004543 1994.048 0.9990463 1.000828 0.889 ns
GDM/non-GDM (SCOPE) 1.0007340 0.0006793 1984.351 0.9994026 1.002067 0.280 ns
GDM/non-GDM (STOP) 1.0007340 0.0006793 1984.351 0.9994026 1.002067 0.280 ns
GH2
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 0.9998217 0.0012642 2132.661 0.9973455 1.002304 0.888 ns
STOP/SCOPE (non-GDM) 0.9999109 0.0006322 2132.661 0.9986719 1.001151 0.888 ns
STOP/SCOPE (GDM) 0.9999109 0.0006322 2132.661 0.9986719 1.001151 0.888 ns
GDM/non-GDM (SCOPE) 1.0004210 0.0009372 2124.039 0.9985847 1.002261 0.653 ns
GDM/non-GDM (STOP) 1.0004210 0.0009372 2124.039 0.9985847 1.002261 0.653 ns
Insulin
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 0.9998688 0.0030312 Inf 0.9939453 1.005828 0.965 ns
STOP/SCOPE (non-GDM) 0.9986023 0.0011978 Inf 0.9962573 1.000953 0.244 ns
STOP/SCOPE (GDM) 1.0012683 0.0027886 Inf 0.9958176 1.006749 0.649 ns
GDM/non-GDM (SCOPE) 0.9972847 0.0024473 Inf 0.9924996 1.002093 0.268 ns
GDM/non-GDM (STOP) 0.9999472 0.0017836 Inf 0.9964576 1.003449 0.976 ns
HOMA.IR
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.0002609 0.0033478 Inf 0.9937207 1.006844 0.938 ns
STOP/SCOPE (non-GDM) 0.9985119 0.0012831 Inf 0.9960002 1.001030 0.246 ns
STOP/SCOPE (GDM) 1.0017516 0.0030963 Inf 0.9957013 1.007839 0.571 ns
GDM/non-GDM (SCOPE) 0.9962530 0.0027774 Inf 0.9908243 1.001712 0.178 ns
GDM/non-GDM (STOP) 0.9994854 0.0018533 Inf 0.9958597 1.003124 0.781 ns
GLUC3_mmol.L
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.0002587 0.0005109 Inf 0.9992579 1.0012605 0.613 ns
STOP/SCOPE (non-GDM) 1.0001294 0.0002554 Inf 0.9996289 1.0006301 0.613 ns
STOP/SCOPE (GDM) 1.0001294 0.0002554 Inf 0.9996289 1.0006301 0.613 ns
GDM/non-GDM (SCOPE) 0.9989546 0.0004852 Inf 0.9980040 0.9999061 0.031 *
GDM/non-GDM (STOP) 0.9989546 0.0004852 Inf 0.9980040 0.9999061 0.031 *

62.2.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

62.2.6 Plots